EXPRESSION OF IMUNOGLOBULIN, GRANZYME-B AND PERFORIN AGAINTS Ag85A AND Ag85B PROTEINS OF MYCOBACTERIUM TUBERCULOSIS IN BALB/C MICE

African Journal of Infectious Diseases (AJID)

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Title EXPRESSION OF IMUNOGLOBULIN, GRANZYME-B AND PERFORIN AGAINTS Ag85A AND Ag85B PROTEINS OF MYCOBACTERIUM TUBERCULOSIS IN BALB/C MICE
 
Creator Fihiruddin, Fihiruddin
Artama, Wayan Tunas
Wibawa, Tri
Mertaniasih, Ni Made
 
Subject Ag85A and Ag85B proteins, Imunoglobulin, granzym B and perforin, TB Vaccine
 
Description Background: Ag85 is a protein that may maintain survival of M.tuberculosis in intracellular parts of host cells and is considered as a virulence factor. The expression of Ag85 protein can stimulate proliferation and differentiation of B cells and T cells in patients with tuberculosis. This research aimed to determine the ability of Ag85A and Ag85B proteins in activating the response of antibodies, granzyme-B and perforin in Balb/c mice.Methods: 25 male Balb/c mice were assigned into five  groups. Group I was treated with adjuvant, group II with BCG vaccine (Biofarma), group III with a combination of BCG and Ag85A, group IV with a combination of BCG and Ag85B and group V with a combination of BCG, Ag85A and Ag85B. Concentrations of Immunoglobulin G, granzym-B and perforin were examined using ELISA and the number of CD8+ T-cells and NK T-cells were checked by flowcytometry.Results: The highest concentration of imunoglobulin G was found in group-V with 62.49±5.4327 ng/ml.  The highest mean number of CD8+ T-cells, NK T-cells, granzyme-B and perforin was found in group–IV with 4.32%, 1.03%, 35.11±1.7789 pg/ml and 6.19±0.2235 pg/ml respectively. The results of One-Way Anova test showed that there were significant differences in immunoglobulin responses, with p <0.05. The expressions of granzym-B and perforin were  higher in mice treated with combination of BCG and recombinant proteins.Conclusions: Ag85 protein can be combined with the BCG vaccine to improve protection against M. tuberculosis infection.
 
Publisher African Traditional Medicine Supporters Initiative (ATHMSI), 7, Road 1, Otunmaiye Square
 
Date 2019-07-03
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
TEXT
 
Format application/pdf
 
Identifier http://journals.athmsi.org/index.php/AJID/article/view/5317
10.21010/Ajid.v13i2.2
 
Source African Journal of Infectious Diseases (AJID); Vol 13 No 2 (2019); 13-20
2505-0419
2006-0165
 
Language eng
 
Relation http://journals.athmsi.org/index.php/AJID/article/view/5317/3191
 
Rights Copyright (c) 2019 Wayan Tunas Artama
http://creativecommons.org/licenses/by/4.0
 

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