EVALUATION OF RADIORESPIROMETRIC TECHNIQUE FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS STRAINS FROM PULMONARY TUBERCULOSIS PATIENTS

LIFE: International Journal of Health and Life-Sciences

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Field Value
 
Title EVALUATION OF RADIORESPIROMETRIC TECHNIQUE FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS STRAINS FROM PULMONARY TUBERCULOSIS PATIENTS
 
Creator Vaidya, Shashikant
Huliyalkar, Vibhavari
Gohil, Auna
Chowdhary, Abhay
Shah, Damayanti
Sengupta, Satya Ranjan
 
Subject Radiorespirometric Method
Visual Growth Method
Mycobacterium Tuberculosis
 
Description A simple and rapid radiorespirometric method, for early detection of tubercle bacilli from sputum samples has been developed. A biphasic liquid scintillation vial system is used for detection of 14CO2 produced by metabolism of acetate 1-2-l4C on glycerol free Lowenstein- Jensen Media (LJM). Total 207 sputum samples were tested by smear, visual growth method (VGM) and radiorespirometric method (RRM) for detection of Mycobacterium tuberculosis (M. tuberculosis) strains. Total 27.54 % sputum samples were scored positive by smear, 21.7% samples by VGM and 33.3 % samples by RRM. There is significant difference between three methods related to sensitivity, specificity, positive and negative predictive value for detection of M. tuberculosis strains. RRM was found to be most sensitive and specific method amongst three. The average detection time by RRM was 9.48 days, compared to 34.47 days by VGM. There is a correlation of number of bacilli in smear and rate of growth in RRM and VGM.Article DOI: https://dx.doi.org/10.20319/lijshls.2015.s11.5571 This work is licensed under the Creative Commons Attribution-Non-commercial 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.
 
Publisher Global Research & Development Services Publishing
 
Date 2015-07-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://grdspublishing.org/index.php/life/article/view/247
10.20319/lijshls.2015.s11.5571
 
Source LIFE: International Journal of Health and Life-Sciences; Vol 1 No 1 (2015): Special Issue
2454-5872
 
Language eng
 
Relation https://grdspublishing.org/index.php/life/article/view/247/1369
 
Rights Copyright (c) 2017 Author(s)
 

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