USE OF GLYCEROL AS CRYOPROTECTANTS IN FREEZING SENTUL CHICKEN SEMEN

Chalaza Journal of Animal Husbandry

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Field Value
 
Title USE OF GLYCEROL AS CRYOPROTECTANTS IN FREEZING SENTUL CHICKEN SEMEN
 
Creator Junaedi, Junaedi
Arifiantini, Raden Iis
Sumantri, Cece
Gunawan, Asep
 
Subject Pemuliaan Ternak, Program Studi Peternakan
frozen semen, chicken Sentul, glycerol
 
Description On the freezing semen chicken cryoprotectants required to overcome the damage of spermatozoa due to cold shock. This study aims to get the best concentrations of cryoprotectants glycerol concentration of 5%, 7% and 9% in freezing Sentul chicken semen. The semen used in this study came from three chickens Sentul and be repeated nine times. Semen was collected by  messase methods for three times a week. Semen was evaluated macroscopic and microscopic. Furthermore spermatozoa diluted with egg yolk and the addition of three concentrations of cryoprotectants glycerol (5%, 7% and 9%). Semen diluted 0:25 ml is packed into straw. Then equilibrated at a temperature of 5°C for two hours. After equilibration to evaluate the motility and viability of spermatozoa. Furthermore, frozen in liquid nitrogen vapor for 10 minutes. Frozen semen is then stored in liquid nitrogen containers with temperature -196°C. After 24 hours, semen is thawed at 37°C for 30 seconds. The results showed that the percentage of sperm motility and viability of frozen semen cock Sentul using glycerol cryoprotectants 5% better P (<0.05) compared with the use of glycerol 7% and 9%. The use of glycerol 5% at this stage of equilibration and storage can reduce the damage of spermatozoa in the semen of chicken Sentul. Neither glycerol 5% could increase recovery rate after thawing
 
Publisher UNIVERSITAS SEMBILANBELAS NOVEMBER KOLAKA
 
Contributor
 
Date 2016-12-10
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://usnsj.com/index.php/CJAH/article/view/CJAH002
10.31327/chalaza.v1i2 December.165
 
Source Chalaza Journal of Animal Husbandry; Vol 1, No 2 December (2016): Chalaza Journal of Animal Husbandry; 6-13
2548-3803
2548-5504
10.31327/chalaza.v1i2 December
 
Language eng
 
Relation http://usnsj.com/index.php/CJAH/article/view/CJAH002/pdf
 
Rights Copyright (c) 2016 Junaedi, Raden Iis Arifiantini, Cece Sumantri, Asep Gunawan
http://creativecommons.org/licenses/by/4.0
 

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