Adição de NaCI em soluções fixadoras - observações histológicas e histoquímicas

Semina: Ciências Sociais e Humanas

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Title Adição de NaCI em soluções fixadoras - observações histológicas e histoquímicas
Adição de NaCI em soluções fixadoras - observações histológicas e histoquímicas
 
Creator Nachi, Arestides Massao
Piza, Ivan Giacomo
 
Subject



 
Description Análise comparativa entre os resultados obtidos após fixação com formol neutro a 10% e com Bouin (convencional) e os resultados obtidos após fixação com essas mesmas soluções acrescidas de NaCI na concentração de 0,7% O orgão-objeto de análise foi a glândula salivar submandibular do rato e os resultados foram avaliados após colorações de H-E e tricrómico de Mallory e, ainda, mediante técnicas Histoquimicas para muco substâncias e proteínas.
A comparative analysis of results obtained with different fixative solutions is presented. The fixatives used were neutral formaldehyde, conventional Bouin, and these same solutions containing 0.7% NaCl. The organ studied under each of these fixative conditions was the submandibular salivary gland of the rat, which was evaluated in parafin sections stained with HE and Mallory as well as by means of histochemical techniques for mucosubstances and proteins. The following conclusions are drawn: 1. An addition of 0.7% NaCl improves both the 10% formaldehyde and the Bouin fixatives; 2. The Bouin-NaCI fixative is good for general morphologic studies but neither for acini nor ductal granules; 3. For microscopic observations of acini and ductal granules the best fixative is the formaldehyde-NaCl; 4. The saline formaldehyde gives the most adequate fixation for histochemical techniques.
 
Publisher Universidade Estadual de Londrina
 
Contributor

 
Date 2004-11-29
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artigo Avaliado pelos Pares
 
Format application/pdf
 
Identifier http://www.uel.br/revistas/uel/index.php/seminasoc/article/view/7262
10.5433/1679-0383.1981v2n8p173
 
Source Semina: Ciências Sociais e Humanas; v. 2, n. 8 (1981); 173-177
1679-0383
1676-5443
 
Language por
 
Relation http://www.uel.br/revistas/uel/index.php/seminasoc/article/view/7262/6414
 

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