Effect of reperfusion injury from distant ischemia to small intestine

Medical Journal of Indonesia

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Field Value
 
Title Effect of reperfusion injury from distant ischemia to small intestine
 
Creator Moenadjat, Yefta; Department of Surgery Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta
Ramdhani, Aris; Department of Surgery Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta
Jeo, Wifanto Saditya; Department of Surgery Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta
Suharto, Wuryantoro; Department of Surgery Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta
Werdhani, Retno Asti; Department of Community Medicine Faculty of Medicine, Universitas Indonesia, Jakarta
 
Subject ileal intestinal villi; ischemia; ischemia-reperfusion injury; ischemic hypothermia; ischemic preconditioning
 
Description BACKGROUND The ileum is the most vulnerable part of the small intestine that plays an important role as the motor of multisystem organ failure. Villous damage is demonstrated after ligation of supply artery in mice; however, there is no study on the ileum after distant ischemic organs. Thus, this study was aimed to find out ileal villous changes following reperfusion injury, the protective effects of ischemic hypothermia and ischemic preconditioning.METHODS An experimental study conducted enrolled 21 subjects of Oryctolagus cuniculus. Ischemia is induced by ligation of the femoral artery for 4 hours. Eight hours after ligation was released, ileum and duodenal specimens were taken through laparotomy. H&E stained specimens were examined for histomorphological changes. Villi change scores, tissue level of hypoxia-inducible factor-1α (HIF-1α), malondialdehyde (MDA), and occludin were statistically analyzed in four treatment groups, namely ischemia, ischemic hypothermia, ischemic preconditioning, and control.RESULTS Intestinal villi changes were found following ischemic-induced arterial ligation. Ileal villi changes showed differences with the duodenum and controls as indicated by the villi damage scores, increased tissue HIF-1α and MDA, and decreased occludin levels. Ileal villi changes in the ischemic and ischemic hypothermia groups showed significant changes with controls; whereas the ischemic preconditioning group showed no significant differences.CONCLUSIONS Ischemia at a distance leads to both histomorphological and biochemical damage of the ileal villi and disrupts the integrity of the intestinal mucosal barrier. In addition, the study showed a protective effect of ischemic preconditioning.
 
Publisher Faculty of Medicine Universitas Indonesia
 
Contributor RSUPN dr. Cipto Mangunkusumo
 
Date 2019-05-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://mji.ui.ac.id/journal/index.php/mji/article/view/1468
10.13181/mji.v28i1.1468
 
Source Medical Journal of Indonesia; Vol 28, No 1 (2019): March; 8-13
2252-8083
0853-1773
10.13181/mji.v28i1
 
Language eng
 
Relation http://mji.ui.ac.id/journal/index.php/mji/article/view/1468/1310
 
Rights Copyright (c) 2019 Yefta Moenadjat, Aris Ramdhani, Wifanto Saditya Jeo, Wuryantoro Suharto, Retno Asti Werdhani
http://creativecommons.org/licenses/by-nc/4.0
 

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