Functional features of neutrophils in subclinical hypothyroidism compared to euthyroid status: Functional features of neutrophils in subclinical hypothyroidism

Annals of Medical Physiology

View Publication Info
 
 
Field Value
 
Title Functional features of neutrophils in subclinical hypothyroidism compared to euthyroid status: Functional features of neutrophils in subclinical hypothyroidism
 
Creator Mohan, Bindu
Damodar, Komaladevi Sampath
 
Subject Candidicidal activity
Chemotaxis
Neutrophils
Phagocytosis
Respiratory burst activity
Subclinical hypothyroidism
Candidicidal activity
Chemotaxis
Neutrophils
Phagocytosis
Respiratory burst activity
Subclinical hypothyroidism
 
Description Neutrophil or polymorphonuclear leukocytes are critical to host defense. They act by migrating (chemotaxis) to site of inflammation or infection, phagocytoze the microorganisms to form phagosome. Reactive Oxygen species and hydrolytic enzymes are released (respiratory burst activity) into this phagosome to kill ingested microorganisms (microbicidal activity). Thyroid hormones are essential for proper differentiation, growth and metabolism of an organism. Increasing evidence indicates that thyroid hormones also play an active role in immunity. Subclinical hypothyroidism is associated with normal thyroid hormone levels with increased thyroid stimulating hormone level. While studies indicate elevated TSH to have some effects on immune functions, there is very little evidence regarding its effect on functional features of neutrophils. In this cross-sectional comparative study, we evaluated the phagocytic, chemotactic, candidacidal, and respiratory burst activity of neutrophils in subclinical hypothyroid and euthyroid subjects. Phagocytosis and metabolic function of the neutrophils was assessed using Nitro-blue tetrazolium test. Chemotaxis, directional movement of the PMNs was measured using the N-Formyl methionyl-leucyl-phenylalanine as chemoattractant. Phagocytic activity was evaluated by exposing leukocytes to candida suspension and counting the number of candida ingested and killed. Microbicidal activity of neutrophils was assessed by the ability of leukocytes to kill Candida albicans in both subclinical hypothyroid and euthyroid subjects. Our study demonstrated that chemotactic and mean phagocytic activity was significantly less in those with subclinical hypothyroidism as compared to euthyroid control group (p value= 0.005 and 0.045 respectively). Respiratory burst activities of both unstimulated & stimulated neutrophils were relatively reduced in subclinical hypothyroidism (p value of 0.08 and 0.07). Candidicidal activity was similar in both groups. We conclude that subclinical hypothyroid individuals having excess of thyroid stimulating hormones are associated altered functional characteristics of neutrophils.
 
Publisher Quench Academy of Medical Education and Research (QAMER) Publishing House
 
Date 2019-02-16
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-Reviewed Article
text
 
Format application/pdf
application/xml
text/html
 
Identifier https://amphysiol.com/index.php/amp/article/view/v3i1.19251
10.23921/amp.2019v3i1.19251
 
Source Annals of Medical Physiology; Vol. 3 No. 1 (2019); 3-9
2456-8422
 
Language eng
 
Relation https://amphysiol.com/index.php/amp/article/view/v3i1.19251/2019_3_9.pdf
https://amphysiol.com/index.php/amp/article/view/v3i1.19251/2019_3_9.xml
https://amphysiol.com/index.php/amp/article/view/v3i1.19251/2019_3_9.html
 
Coverage Worldwide
 
Rights Copyright (c) 2019 Quench Academy of Medical Education and Research (QAMER)
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library