Functional features of neutrophils in subclinical hypothyroidism compared to euthyroid status: Functional features of neutrophils in subclinical hypothyroidism
Annals of Medical Physiology
View Publication Info| Field | Value | |
| Title |
Functional features of neutrophils in subclinical hypothyroidism compared to euthyroid status: Functional features of neutrophils in subclinical hypothyroidism
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| Creator |
Mohan, Bindu
Damodar, Komaladevi Sampath |
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| Subject |
Candidicidal activity
Chemotaxis Neutrophils Phagocytosis Respiratory burst activity Subclinical hypothyroidism Candidicidal activity Chemotaxis Neutrophils Phagocytosis Respiratory burst activity Subclinical hypothyroidism |
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| Description |
Neutrophil or polymorphonuclear leukocytes are critical to host defense. They act by migrating (chemotaxis) to site of inflammation or infection, phagocytoze the microorganisms to form phagosome. Reactive Oxygen species and hydrolytic enzymes are released (respiratory burst activity) into this phagosome to kill ingested microorganisms (microbicidal activity). Thyroid hormones are essential for proper differentiation, growth and metabolism of an organism. Increasing evidence indicates that thyroid hormones also play an active role in immunity. Subclinical hypothyroidism is associated with normal thyroid hormone levels with increased thyroid stimulating hormone level. While studies indicate elevated TSH to have some effects on immune functions, there is very little evidence regarding its effect on functional features of neutrophils. In this cross-sectional comparative study, we evaluated the phagocytic, chemotactic, candidacidal, and respiratory burst activity of neutrophils in subclinical hypothyroid and euthyroid subjects. Phagocytosis and metabolic function of the neutrophils was assessed using Nitro-blue tetrazolium test. Chemotaxis, directional movement of the PMNs was measured using the N-Formyl methionyl-leucyl-phenylalanine as chemoattractant. Phagocytic activity was evaluated by exposing leukocytes to candida suspension and counting the number of candida ingested and killed. Microbicidal activity of neutrophils was assessed by the ability of leukocytes to kill Candida albicans in both subclinical hypothyroid and euthyroid subjects. Our study demonstrated that chemotactic and mean phagocytic activity was significantly less in those with subclinical hypothyroidism as compared to euthyroid control group (p value= 0.005 and 0.045 respectively). Respiratory burst activities of both unstimulated & stimulated neutrophils were relatively reduced in subclinical hypothyroidism (p value of 0.08 and 0.07). Candidicidal activity was similar in both groups. We conclude that subclinical hypothyroid individuals having excess of thyroid stimulating hormones are associated altered functional characteristics of neutrophils.
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| Publisher |
Quench Academy of Medical Education and Research (QAMER) Publishing House
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| Date |
2019-02-16
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| Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion Peer-Reviewed Article text |
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| Format |
application/pdf
application/xml text/html |
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| Identifier |
https://amphysiol.com/index.php/amp/article/view/v3i1.19251
10.23921/amp.2019v3i1.19251 |
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| Source |
Annals of Medical Physiology; Vol. 3 No. 1 (2019); 3-9
2456-8422 |
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| Language |
eng
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| Relation |
https://amphysiol.com/index.php/amp/article/view/v3i1.19251/2019_3_9.pdf
https://amphysiol.com/index.php/amp/article/view/v3i1.19251/2019_3_9.xml https://amphysiol.com/index.php/amp/article/view/v3i1.19251/2019_3_9.html |
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| Coverage |
Worldwide
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| Rights |
Copyright (c) 2019 Quench Academy of Medical Education and Research (QAMER)
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