LC–MS/MS Method for Quantitation of Hydrochlorothia-zide and Nifedipine in Human Plasma

ABC Research Alert

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Title LC–MS/MS Method for Quantitation of Hydrochlorothia-zide and Nifedipine in Human Plasma
 
Creator Ongas, Martin O.; Center for Research in Therapeutic Sciences, Strathmore University, Nairobi
Kokwaro, Gilbert; Center for Research in Therapeutic Sciences and Institute of Healthcare Management, Strathmore University Nairobi
Oloo, Florence; Center for Research in Therapeutic Sciences, Strathmore University, Nairobi
Kirimi, Caroline K.; Center for Research in Therapeutic Sciences, Strathmore University, Nairobi
Mkonje, Amina; Center for Research in Therapeutic Sciences, Strathmore University, Nairobi
Otieno, Fredrick; Department of Clinical Medicine and Therapeutics, University of Nairobi
Muasya, Christine; Department of Clinical Pharmacology, University of Nairobi,
Omollo, Raymond; Center for Research in Therapeutic Sciences, Strathmore University and KEMRI-Centre for Clinical Research, Nairobi
Siddondo, Bethseba; Center for Research in Therapeutic Sciences, Strathmore University, Nairobi
Midamba, Brian; Center for Research in Therapeutic Sciences, Strathmore University, Nairobi
Cole, Andrew O.; Center for Research in Therapeutic Sciences, Strathmore University, Nairobi
Ogola, Elijah S. N.; Department of Clinical Medicine and Therapeutics, University of Nairobi
Aman, Rashid; Center for Research in Therapeutic Sciences, Strathmore University and African Centre for Clinical Trials (ACCT), Nairobi
Ogutu, Bernhards; Center for Research in Therapeutic Sciences, Strathmore University and KEMRI-Centre for Clinical Research
 
Subject LC-MS/MS; Hydrochlorothiazide; Nifedipine; Hypertension; Human plasma; Non-communicable diseases.
 
Description We have developed and validated a novel, sensitive, selective and reproducible reversed-phase  high-performance liquid chromatography method coupled with electrospray ionization mass spectrometry (HPLC–ESI-MS/MS) for the simultaneous quantitation of hydrochlorothiazide (HCTZ) and nifedipine (NFP) from only 200 µL of human plasma. Diazepam (DZP) was used as an internal standard (IS). The analytes were extracted by a liquid-liquid extraction  procedure with ethyl acetate-dichloromethane and separated on a reversed-phase Polaris 5 C18 Aanalytical column using a mobile phase composed of methanol containing 0.1% (v/v) formic acid and 5mM aqueous ammonium formate pH 6.0, delivered at a flow-rate of 300µL/min. Multiple reaction monitoring was performed in the negative ion mode using the transition m/z296.1→m/z205.2 (HCTZ), positive ion mode transitions m/z347.2→m/z 315.1 (NFP) and m/z285.0→m/z 193.2 (DZP) to quantify the drugs. Calibration curves in spiked plasma were linear (r2 ≥ 0.9953) from 5–2000 ng/mL for HCTZ and 5 – 400 ng/mL for NFP with a lower limit of quantification (LLOQ) of 5 ng/mL for both drugs. The intra- and inter- assay precisions (coefficient of variation) were less than 9% and the mean extraction recoveries were 98.1% (HCTZ), 99.5% (NFP) and 93.8% for the IS (DZP). The validated method was successfully applied to a limited population pharmacokinetics study of HCTZ and NFP among patients on hypertension care.
 
Publisher Asian Business Consortium
 
Contributor
 
Date 2018-11-18
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier https://journals.abc.us.org/index.php/abcra/article/view/1135
10.18034/abcra.v6i3.1135
 
Source ABC Research Alert; Vol 6, No 3 (2018): September-December 2018 Issue; Kenya
10.18034/abcra.v6i3
 
Language eng
 
Relation https://journals.abc.us.org/index.php/abcra/article/view/1135/1021
 
Rights Copyright (c) 2018 Martin O. Ongas, Gilbert Kokwaro, Florence Oloo, Caroline K. Kirimi, Amina Mkonje, Fredrick Otieno, Christine Muasya, Raymond Omollo, Bethseba Siddondo, Brian Midamba, Andrew O. Cole, Elijah S. N. Ogola, Rashid Aman, Bernhards Ogutu
http://creativecommons.org/licenses/by-nc/4.0
 

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