Mangifera indica L. Leaves Extract Induced Intrinsic Apoptotic Pathway in MCF-7 Cells by Decreasing Bcl-2 Expression and Inducing Bax Expression

Indonesian Journal of Cancer Chemoprevention

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Title Mangifera indica L. Leaves Extract Induced Intrinsic Apoptotic Pathway in MCF-7 Cells by Decreasing Bcl-2 Expression and Inducing Bax Expression
 
Creator Fitriasih, Fitriasih
Komariyah, Siti Mega
Sandra, Ferry
Pratiwi, Nenni
Hidayati, Devi Nisa
 
Description Extract of Mangifera indica L. has been investigated to have cytotoxic activity against MCF-7 cells.  Current research was conducted to determine the cytotoxic activity of methanol extract of M. indica leaves (MEML) on MCF-7 breast cancer cells and to investigate on the MEML's effect on Bcl-2 and Bax expressions. M. indica leaves were extracted using soxhletation with methanol. The cytotoxic test was perfomed using MTT assay with various MEML concentrations: 15.625, 31.25, 62.5, 125, 250 and 1000 μg/mL. Meanwhile, Bax and Bcl-2 expressions of MCF-7 breast cancer cells were detected with immunocytochemical staining after treatment of MEML with concentrations of ¼ IC50, ½ IC50 and IC50. The results showed that IC50 of cytotoxic activity was 308.12 µg/mL. MEML could influence Bax and Bcl-2 protein expressions. After treatment of MEML, Bcl-2 expression was decreased while Bax expression was increased.Keywords : Mangifera indica L., Cytotoxic, Bax, Bcl-2, MCF-7
 
Publisher Indonesian Society for Cancer Chemoprevention
 
Contributor Ministry of Research, Technology and Higher Education of the Republic of Indonesia
 
Date 2019-02-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier https://ijcc.chemoprev.org/index.php/ijcc/article/view/243
10.14499/indonesianjcanchemoprev10iss1pp1-6
 
Source Indonesian Journal of Cancer Chemoprevention; Vol 10, No 1 (2019); 1-6
2355-8989
2088-0197
 
Language eng
 
Relation https://ijcc.chemoprev.org/index.php/ijcc/article/view/243/175
 

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